碳/氧化鋅納米線陣列/泡沫石墨烯電化學檢測葉酸

王寶; 林軒宇; 黃碩; 高鑫; 姚龍輝; 岳紅彥

doi:10.13374/j.issn2095-9389.2017.11.006

碳/氧化鋅納米線陣列/泡沫石墨烯電化學檢測葉酸

doi: 10.13374/j.issn2095-9389.2017.11.006

王寶¹,
林軒宇¹,
黃碩^1,2,
高鑫¹,
姚龍輝¹,
岳紅彥^1, ,

1) 哈爾濱理工大學材料科學與工程學院, 哈爾濱 150040
2) 哈爾濱醫科大學附屬第一醫院神經內科, 哈爾濱 150001

基金項目:

哈爾濱理工大學青年拔尖創新人才培養計劃資助項目(201604)

黑龍江博士后科研啟動基金資助項目(LBH-Q14117)

哈爾濱市應用技術研究與開發資助項目(2016RAQXJ185)

國家留學人員科技活動資助項目(2015192)

黑龍江省自然科學基金資助項目(LC2015020)

詳細信息

中圖分類號: TB332;TP212.3
計量
- 文章訪問數: 825
- HTML全文瀏覽量: 377
- PDF下載量: 13
- 被引次數: 0
出版歷程
- 收稿日期: 2017-03-07

Electrochemical determination of folic acid using carbon/ZnO nanowire arrays/graphene foam

1) School of Material Science and Engineering, Harbin University of Science and Technology, Harbin 150040, China
2) Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China

摘要

摘要: 通過化學氣相沉積法制備三維(3D)泡沫石墨烯(GF),然后利用水熱合成法在泡沫石墨烯表面生長氧化鋅納米線陣列(ZnO NWAs),再利用化學氣相沉積法在其表面沉積碳(C),得到碳/氧化鋅納米線陣列/泡沫石墨烯(C/ZnO NWAs/GF)復合材料.用該復合材料做電極,采用電化學方法檢測葉酸(FA).結果表明,三維泡沫石墨烯具有和模板泡沫鎳一樣的三維孔狀結構,ZnO NWAs均勻且垂直地生長在泡沫石墨烯表面,碳沉積在ZnO NWAs表面.在線性范圍為0~60 μmol·L^-1內,C/ZnO NWAs/GF電極檢測FA時,靈敏度為0.13 μA·μmol^-1·L,且在尿酸(UA)干擾下檢測FA具有良好的選擇性.C/ZnONWAs/GF電極有良好的穩定性和重復性.
- 泡沫石墨烯 /
- 氧化鋅納米線陣列 /
- 碳 /
- 電化學生物傳感器 /
- 葉酸 /
- 尿酸
Abstract: Three-dimensional (3D) graphene foam (GF) was synthesized by chemical vapor deposition (CVD). Following this, ZnO nanowire arrays (ZnO NWAs) were grown on the 3D GF by hydrothermal synthesis. Finally, carbon (C) was deposited on the surface of ZnO NWAs by CVD to obtain a C/ZnO NWAs/GF hybrid. This composite was used to detect the presence of folic acid. The experimental results show that the GF inherits the 3D macroporous structure of nickel foam and that the ZnO NWAs are uniformly and vertically grown on the 3D GF. The length and diameter of the ZnO nanowires are~50 nm and 2 μm, respectively. Carbon is deposited on the surface of ZnO NWAs, and the C/ZnO NWAs/GF is used as a working electrode to detect folic acid (FA) using an electrochemical method. The experimental results show that the sensitivity of the electrode for FA is 0.13 μA·μmol^-1·L and it has good selectivity for detecting FA in the presence of uric acid. The electrode also has excellent reproducibility and stability.
- graphene foam /
- ZnO nanowire arrays /
- carbon /
- electrochemical biosensor /
- folic acid /
- uric acid

HTML全文

參考文獻(24)

[1]	Asaikkutti A, Bhavan P S, Vimala K. Effects of different levels of dietary folic acid on the growth performance, muscle composition, immune response and antioxidant capacity of freshwater prawn, Macrobrachium rosenbergii. Aquaculture, 2016, 464:136
[2]	Cheung R H F, Morrison P D, Small D M, et al. Investigation of folic acid stability in fortified instant noodles by use of capillary electrophoresis and reversed-phase high performance liquid chromatography. J Chromatogr A, 2008, 1213(1):93
[3]	Phillips K M, Ruggio D M, Haytowitz D B. Folate composition of 10 types of mushrooms determined by liquid chromatography-mass spectrometry. Food Chem, 2011, 129(2):630
[4]	Kirsch S H, Knapp J P, Herrmann W, et al. Quantification of key folate forms in serum using stable-isotope dilution ultra performance liquid chromatography-tandem mass spectrometry. J Chromatogr B, 2010, 878(1):68
[5]	Shishehbore M R, Sheibani A, Haghdost A. Kinetic spectrophotometric method as a new strategy for the determination of vitamin B9 in pharmaceutical and biological samples. Spectrochim Acta A, 2011, 81(1):304
[6]	Akhtar M J, Khan M A, Ahmad I. Identification of photoproducts of folic acid and its degradation pathways in aqueous solution. J Pharm Biomed Anal, 2003, 31(3):579
[7]	Zhang B T, Zhao L X, Lin J M. Determination of folic acid by chemiluminescence based on peroxomonosulfate-cobalt (Ⅱ) system. Talanta, 2008, 74(5):1154
[8]	Blanco C C, Carretero A S, Gutierrez A F, et al. Fluorometric determination of folic acid based on its reaction with the fluorogenic reagent fluorescamine. Anal Lett, 1994, 27(7):1339
[9]	Arcot J, Shrestha A K, Gusanov U. Enzyme protein binding assay for determining folic acid in fortified cereal foods and stability of folic acid under different extraction conditions. Food Control, 2002, 13(4-5):245
[10]	Chen A C, Shah B. Electrochemical sensing and biosensing based on square wave voltammetry. Anal Methods, 2013, 5:2158
[11]	KHan M, Tahir M N, Adil S F, et al. Graphene based metal and metal oxide nanocomposites:synthesis, properties and their applications. J Mater Chem A, 2015, 3:18753
[12]	Yue H Y, Huang S, Chang J, et al. ZnO nanowire arrays on 3D hierachical graphene foam:biomarker detection of Parkinson's disease. Acs Nano, 2014, 8(2):1639
[13]	Liu X W, Hu Q Y, Wu Q, et al. Aligned ZnO nanorods:a useful film to fabricate amperometric glucose biosensor. Colloid Surface B, 2009, 74(1):154
[14]	Ma S W, Liao Q L, Liu H S, et al. An excellent enzymatic lactic acid biosensor with ZnO nanowires-gated AlGaAs/GaAs high electron mobility transistor. Nanoscale, 2012, 4:6415
[15]	Ma S W, Zhang X H, Liao Q L, et al. Enzymatic lactic acid sensing by In-doped ZnO nanowires functionalized AlGaAs/GaAs high electron mobility transistor. Sens Actuators B:Chem, 2015, 212:41
[16]	Vayssieres L. Growth of arrayed nanorods and nanowires of ZnO from aqueous solutions. Adv Mater, 2003, 15(5):464
[17]	Ahmad M, Zhu J. ZnO based advanced functional nanostructures:synthesis, properties and applications. J Mater Chem, 2011, 21:599
[18]	Zhan H L, Garrett D J, Apollo N V, et al. Direct fabrication of 3D graphene on nanoporous anodic alumina by plasma-enhanced chemical vapor deposition. Sci Rep, 2016, 6:19822.
[19]	Chen Z P, Ren W C, Gao L B, et al. Three-dimensional flexible and conductive interconnected graphene networks grown by chemical vapour deposition. Nat Mater, 2011, 10(6):424
[20]	Babaei A, Babazadeh M. A selective simultaneous determination of levodopa and serotonin using a glassy carbon electrode modified with multiwalled carbon nanotube/chitosan composite. Electroanalysis, 2011, 23(7):1726
[21]	Taei M, Jamshidi M. Highly selective determination of ascorbic acid, epinephrine,and uric acid by differential pulse voltammetry using poly(Adizol Black B)-modified glassy carbon electrode. J Solid State Electrochem, 2014, 18(3):673
[22]	O'Shea T J, Garcia A C, Blanco P T, et al. Electrochemical pretreatment of carbon fibre microelectrodes for the determination of folic acid. J Electroanal Chem Interf Electrochem, 1991, 307(1-2):63
[23]	Beitollahi H, Hamzavi M, Torkzadeh-Mahani M. Electrochemical determination of hydrochlorothiazide and folic acid in real samples using amodified graphene oxide sheet paste electrode. Mater Sci Eng C, 2015, 52:297
[24]	Mazloum-Ardakani M, Beitollahi H, Amini M K, et al. Simultaneous and selective voltammetric determination of epinephrine, acetaminophen and folic acid at a ZrO₂ nanoparticles modified carbon paste electrode. Anal Methods, 2011, 3:673