Heterologous expression and purification of the ligand binding domain of estrogen receptor β

SHI Guoqing; GUO Yanfeng; WEI Wei; SONG Qing; ZHANG Huai; ZHONG Guangrong

doi:10.13374/j.issn1001-053x.2007.s2.087

Volume 29 Issue S2

Aug. 2021

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Article Navigation > Chinese Journal of Engineering > 2007 > 29(S2): 208-211

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

Citation:

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

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Heterologous expression and purification of the ligand binding domain of estrogen receptor β

doi: 10.13374/j.issn1001-053x.2007.s2.087

Applied Science School, University of Science and Technology Beijing, Beijing 100083, China

Received Date: 2007-08-15
Available Online: 2021-08-16

Abstract

Abstract

In order to investigate the interaction between environmental chemicals and estrogen receptors,the ligand binding domain of human estrogen receptor β(hERβ-LBD) was expressed in Escherichia coli.Based on the optimized expression conditions(IPTG,0.2 mmol·L^-1;culture temperature,25℃;time of induction,8 h),after purified by affinity chromatography,the production of hERβ-LBD can be reached to 236 mg·L^-1.
- estrogen receptor,
- ligand binding domain,
- expression,
- purification